Analysis Note

ControlHeLa whole cell extract, human neuroblastoma SH-SY5Y cells

Application

Research CategoryStem Cell Research

Research Sub CategoryNeural Stem Cells

Immunoblotting: 1:500-1:1000

Immunocytochemistry: 1:100-1:300 on COS7, HeLa, 293 and SK-N-MC cell lines.

Optimal working dilutions must be determined by the end user.

Anti-Mitochondria Antibody is an antibody against Mitochondria for use in WB & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Presenilin 1 is a protein found in brain cells. Presenilin 1 has a major influence on the risk of contracting alzheimer′s disease. Its function is unknown. However, it may be involved in protein production and trafficking, especially during early development. More than 40 different mutations have been found in the presenilin 1 gene, and these mutations associate with early-onset familial alzheimer′s disease. Mutations in presenilin 1 tend to elevate levels of amyloid beta in the blood, cerebrospinal fluid, and brains of those affected by mutations

Immunogen

Presenilin 1 peptide conjugated to KLH.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit serum preabsorbed against immunogen peptide. Liquid. Contains 0.01% sodium azide.

Specificity

Mitochondria. Recognizes a 62 kD protein of immunoblots of subcellular fractions enriched in mitochondrial fractions.



The antibody shows a moderate titer by ELISA to the immunogen peptide but does not immunoblot any expected presenilin 1 bands. Immunocytochemistry with the serum revealed a mitochondria-like staining that was still present after exhaustive preabsorption with the peptide. The staining co-localizes with CHEMICON′s anti-mitochondria antibody MAB1273.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.

Target description

62 kDa